An unbalanced load may present a risk to both the machine and to persons nearby. Conduct rotor or safety cup/bucket cleanup in nearest biosafety cabinet or fume hood, depending on hazard. Maintain log book: For all high speed centrifuges and ultracentrifuges include run dates, durations, speeds, total rotor revolutions, and notes on rotor condition. Retire rotors after manufacturer’s recommended life span except where annual stress test demonstrates absence of structural flaws. 2. Review this document and supply the information required in order to make it specific to your laboratory. Click here for more information. Balance centrifuge: Follow manufacturer’s instructions for proper centrifuge balancing steps. Follow spill, exposure, and incident reporting instructions. Public health and clinical laboratory staff, safety professionals and persons interested in safe use of centrifuges. Another potential hazard is the possible aerosolization of harmful samples during centrifuge operation. Follow the directions on the WorkConnections website Forms Instructions to obtain proper medical treatment and follow-up. This course is approved for 0.5 contact hours. You will be subject to the destination website's privacy policy when you follow the link. When operating the centrifuge, proper eye protection (glasses/goggles), gloves, lab coat, and close-toe footwear should be worn. Do not decant or pour off supernatant. The following information may be integrated into a lab-specific standard operating procedure (SOP) for centrifuge use. 1500 East Medical Center Drive, Ann Arbor (734) 764-8021 Do not leave centrifuge until full operating speed is reached and it appears to be running safely without incident. Hours of Operation. C. Open sealable tubes/safety cups/rotors: Wear appropriate PPE and open inside fume hood or biosafety cabinet, depending on hazard. Open centrifuge tubes that contain biohazardous specimens inside a BSC with the tube pointed away from you. All personnel are required to complete the General Laboratory Safety Training session (BLS025w or equivalent) via OSEH’s My LINC website. Ensure gloves are compatible with hazard(s). CDC twenty four seven. Ensure safety cups/buckets are attached correctly and able to move freely. Saving Lives, Protecting People, Virtual Reality Laboratory Training Partner Toolkit, Competency Guidelines for Laboratory Professionals, U.S. Department of Health & Human Services, Identify common types of centrifuges used in laboratories, Describe the potential hazards associated with centrifuge use, Identify control measures to minimize exposure to centrifuge hazards, Identify safe work practices for centrifuge use, Describe what to do in the event of an emergency. A centrifuge is a device that uses centrifugal force to separate component parts of a liquid or fluid (and even gases). Gloves should be appropriate for the present hazard. Do not use centrifuge again until inspected by qualified service technician. UMHS Emergency Department -- after clinic hours or on weekends Use tongs or forceps to avoid contact with sharp-edged debris. C380 Med Inn building Physical hazards: Mechanical failure due to mechanical stress, metal fatigue, and corrosion of the rotor over time 2 I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Follow manufacturer’s filling limits for tubes. Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website. C. Inspect centrifuge: Ensure tubes are rated for intended use (speed, temperature, and chemical resistance). If a specific procedure does not allow for this, contact Occupational Safety and Environmental Health (OSEH) at (734) 647-1143 for guidance. The Charles Woodson Children's Clinical Research Laboratory requires that all users have undergone appropriate OSEH or equivalent training prior to gaining access to the lab (See detailed instructions in the Woodson Lab Core section). University Health Services -- University students (non-life threatening conditions) For radioactive materials, request radiation safety survey and signage before disposal of centrifuge. At the same time, objects that are less dense are displaced and move to the centre. Turn off centrifuge immediately and unplug power cord. Ensure rotor and safety cups/buckets are free of cracks and deformities. B. In accordance with this document, laboratories should use appropriate controls and personal protective equipment when using centrifuges. In a laboratory centrifuge that uses sample tubes, the radia… Mon-Fri 7:30 am - 4:30 pm Wear appropriate PPE: Including safety eyewear, gloves, lab coat, and appropriate street clothing (i.e., long pants and closed-toe shoes). After hours - go to UM Hospital Emergency Dept. Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm Topics covered include major parts of a centrifuge, types of centrifuges, potential hazards, how to work safely with a centrifuge, and what to do if there is an emergency. A certified biological safety cabinet (class I or II) is the primary barrier to protect worker from aerosols if working with RG2 or higher agents. U-M Occupational Health Services -- Campus Employees Do not overfill or underfill tubes. Topics covered include major parts of a centrifuge, types of centrifuges, potential hazards, how to work safely with a centrifuge, and what to do if there is an emergency. Attach note on centrifuge describing what has been done. TREATMENT FACILITIES: A. This basic-level eLearning course provides information on the safe use of centrifuges. B. Complete the OSEH Laboratory Incident and Near-Miss Report form. Ensure tubes are rated for intended use (speed, temperature, and chemical resistance). A. Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. Stop run: Ensure centrifuge comes to complete stop before opening cover. A. Register with the University of Michigan Emergency Alert System via Wolverine Access. As the name centrifuge suggests, these machines use centrifugal force to separate substances. Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Keep centrifuge cover closed for at least 30 minutes to reduce aerosolization of hazardous material. When centrifuging hazardous materials, wait at least 10 minutes after run to allow aerosols to settle before opening centrifuge. Confirm rotor is balanced. Complete lab-specific training for the centrifuge. Avoid abrasive wire brushes for cleaning. 1. I have read and understand the above SOP. Equipment repair and adjustments shall only be conducted by qualified service technicians. A. B. This means that lab managers can improve centrifuge safety at their organizations by refining operating and maintenance procedures and emphasizing staff training. The centrifuge must be loaded carefully balanced. To prevent rotor failure, do not exceed maximum speed and maximum mass limits for the rotor. If centrifuging hazardous materials, care should be taken to use tightly capped tubes and/or sealable safety cups or rotors that can be loaded and unloaded in a fume hood or biosafety cabinet, depending on the hazard.